ABSTRACT
Targeted proteomics by selected reaction monitoring on triple quadrupole mass spectrometers is a widely used strategy to quantify multiple proteins in complex body fluids like serum or in tissue. It was positioned as the method of the year by Nature Methods in 2012, stressing its importance in biomarker research. Although a highly selective method, there are several practical considerations that hinder its widespread use in research and diagnostics. Here, we review the status of target proteomics methodologies and highlight several developments in sample preparation and mass spectrometry that potentially bring targeted proteomics to the next level.
