Fluorescence Lifetime Imaging and Metrology for Biomedicine

Authored by: Talbot Clifford , McGinty James , McGhee Ewan , Owen Dylan , Grant David , Kumar Sunil , De Beule Pieter , Auksorius Egidijus , Manning Hugh , Galletly Neil , Treanor Bebhinn , Kennedy Gordon , Peter M.P. Lanigan , Munro Ian , Daniel S. Elson , Magee Anthony , Davis Dan , Neil Mark , Stamp Gordon , Dunsby Christopher , French Paul

Handbook of Photonics for Biomedical Science

Print publication date:  May  2010
Online publication date:  May  2010

Print ISBN: 9781439806289
eBook ISBN: 9781439806296
Adobe ISBN:

10.1201/9781439806296-c6

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Abstract

This chapter describes recent advances in the development of fluorescence lifetime imaging and related multidimensional fluorescence imaging techniques together with their application to biological tissue and cell cultures. It aims to outline the principles underlying the main fluorescence imaging modalities and describes recent technological advances, with a particular emphasis on fluorescence lifetime imaging. The application of such technology to label-free imaging of biological tissue is then presented with examples from our work at Imperial College London. Applications to imaging molecular cell biology are then reviewed and a brief perspective of future developments is then presented.

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