Abstract

This chapter describes recent advances in the development of fluorescence lifetime imaging and related multidimensional fluorescence imaging techniques together with their application to biological tissue and cell cultures. It aims to outline the principles underlying the main fluorescence imaging modalities and describes recent technological advances, with a particular emphasis on fluorescence lifetime imaging. The application of such technology to label-free imaging of biological tissue is then presented with examples from our work at Imperial College London. Applications to imaging molecular cell biology are then reviewed and a brief perspective of future developments is then presented.