ABSTRACT
Several milestones in biology have been achieved since the first publication of the Handbook of Molecular and Cellular Methods in Biology and Medicine. This is true particularly with respect to genome-level sequencing of higher eukaryotes, the invention of DNA microarray technology, advances in bioinformatics, and the development of RNAi technology
DNA-Based Technology. Isolation and Purification of DNA. DNA Cloning Strategies. Polymerase Chain Reaction Methodologies. Preparation of Nucleic Acid Probes.Southern Blot Hybridization. Genomic DNA Libraries. DNA Sequencing and Analysis. RNA-Based Technology. Isolation and Purification of RNA. RT-PCR and qRT-PCR Methodologies. Preparation of Nucleic Acid Probes. Northern Blot Hybridization. cDNA Libraries. Differential Display. Localization of RNA Expression. Isolation and Purification of Proteins. Western Blot Hybridization. Localization of Protein Expression. DNA Footprinting and Gel Retardation Assay. Protein-Protein and Protein-Ligand Interactions. Metabolites. Isolation and Purification of Metabolites. Analysis of Metabolites. Genomics, Proteomics, and Metabolomics. Bioinformatics and Data Analysis. Microarray Platforms. Microarray Data Collection. Microarray Data Analysis. Proteomics: Data Collection and Analysis. Metabolomics Data Collection and Analysis. Common Protocols on Membrane Lipid Analysis and Lipidomics. Integrated Functional Genomics. Manipulation of Biological Systems. Inhibition of Gene Expression. Inhibition of Protein Synthesis. Gene Transfer and Expression in Animal Cells. Methods of Stem Cell Lineage Analysis In Situ and in Cell Culture. Tissue Engineering of Fully Differentiated Tissue: Lessons from Work on Cardiac Myocytes. Plant Tissue and Cell Culture. Gene Transfer and Expression in Plants. Macromolecular Analyses. Microscopy: Light and Confocal. Microscopy: Scanning Electron, Environmental Scanning Electron, and Transmission Electron. Laser Capture Microdissection and Whole Genome Amplification. Index.
