Analysis of Bioactive Peptides and Proteins

Authored by: Prithy Rupa , Yoshinori Mine

Handbook of Analysis of Active Compounds in Functional Foods

Print publication date:  January  2012
Online publication date:  January  2012

Print ISBN: 9781439815885
eBook ISBN: 9781439815908
Adobe ISBN:

10.1201/b11653-8

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Abstract

Advances in the research field of bioactive proteins and peptides are driven by a molecular understanding of biological processes and various analytical techniques are a critical component of this understanding. In the past, biochemical functional analysis of peptide and protein activity was focused on the analysis of single molecules. Different up-to-date methods, including peptide synthesis and immunochemistry, have been applied to the characterization of bioactive peptides recently. Although this approach has served well, the rapid pace of discovery of new gene products by large-scale genomic and proteomic initiatives has necessitated the design of alternative strategies for analyzing protein and peptide function as compared to traditional analytical methods. Bioactive peptides have been studied by means of the following investigation techniques: establishment of an assay system to determine biological activity, hydrolysis of proteins by digestive enzymes, the isolation of peptides and the determination of the structures, and the synthesis of peptides for the verification of activity. Bioactive peptides usually contain 3–20 amino acid residues per molecule.

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